SIRT1 and obesity due to melanocortin 4 receptor deficiency: Furthermore, the tea mixture had a significant effect on the mRNA expression of p-AMPK, Sirt-1, TNF-α, ADD1/SREBP-1c, and PPAR-γ, as it increased the mRNA expression of p-AMPK, Sirt-1, and PPAR-γ, all of which induce lipolysis, while also decreasing the mRNA expressions of TNF-α and ADD1/SREBP-1c, inhibiting the inflammation associated with obesity and adipogenesis.