In another example, the anticancer ability of CK was examined in the HER2-positive breast cancer cell line (SKBR3) and HER2-negative breast cancer cell line (MDA-MB-231) via 3-(4-5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide assays, propidium iodide, annexin V staining, and morphological changes. This evidence concerns the gene ANXA5 and breast cancer.