Compound 12 suppressed the expression of NLRP3 inflammasome (reported to be well associated with NF-κB signal transduction) and its modulators, such as TXNIP and ACS, both in human RA FLSs and CIA mice, thereby decreasing cleaved caspase-1 levels; thus, anti-inflammatory effects of 12 could be related to the inhibition of NLRP3 inflammasome signaling. Here, NFKB1 is linked to rheumatoid arthritis.