Reduction of iNOS expression, inhibition of IL-1β, IL-6 and TNF-α secretion and antagonization of ROS production in vitroReduction of arthritis incidence, suppression of mRNA expression of IL-1β and amelioration of arthritis score, paw edema and bone erosion in CIA miceMechanistic in vitro studies showed that 16 downregulated Keap1 protein expression and upregulated that of Nrf2, HO-1, NQO1 and p62, in time- and dose-dependent manners. The gene discussed is NOS2; the disease is Arthritis.