We investigated the bile ducts regarding their immunostainings for IRF3 and found out that the number of bile ducts with strong immunopositivity for IRF3, namely staining intensity of +3, was in the NASH patients significantly higher than in the NAFL group (p = 0.027; Figure 3A,B,D). Here, IRF3 is linked to metabolic dysfunction-associated steatohepatitis.