To test whether Adcyap1 and Adcyap1r1 mRNAs, moreover the PAC1R protein content of the brain is affected in the rotenone model of PD, RNAscope ISH and immunofluorescent labeling were carried out in the CPu, GP, EP, SN and EWcp. Here, ADCYAP1R1 is linked to Parkinson disease.