Ingenuity Pathway Analysis (IPA) of these identified directly up-regulated genes (log2FC > 1.2) with enriched H3K27Ac engagement revealed the S100 family, STAT3, and tumor microenvironment pathways to be enriched in both 10A and DCIS ANCO1 knockdown cells (Supplementary Table S7), indicating a direct impact of ANCO1 loss on these pathways in different stages of breast cancer progression. The gene discussed is STAT3; the disease is breast cancer.