ANCO1-regulated peaks had a significantly increased overlap with breast cancer enhancer peaks compared to ANCO1-independent ones which were present in the shCTRL group and did not change in response to ANCO1 knockdown (p < 0.0001 for both 10A and DCIS lines, chi-squared test) (Figure 4B). The gene discussed is ANKRD11; the disease is ductal breast carcinoma in situ.