We demonstrated how the knockdown of circAFF1 can alter the migration capabilities of RH4 and RH30 cells, representative cell lines for alveolar rhabdomyosarcoma (ARMS), through the regulation of the cell adhesion programme genes, underlining the importance of this circRNA as a partial suppressor of a hallmark of cancer, such as the activation of cell migration and metastasis [44]. Here, RHD is linked to rhabdomyosarcoma.