In the in vitro experiments conducted in this study, we first verified that RBM10 was expressed in the nucleus and cytoplasm of lung adenocarcinoma A549 cells in equal amounts by using an immunofluorescence method, and qRT-PCR showed that the expression of RBM10 in the A549 cell line was significantly lower than that in the control group. This evidence concerns the gene RBM10 and lung adenocarcinoma.