In line with this, Roudi et al. evaluated, through immunohistochemical staining, the expression of CSCs markers OCT4 and NANOG, reporting a trend between low OCT4 expression and absence of metastasis or lymph node involvement, which could indicate that increased OCT4 expression would contribute to the malignant behavior of CRC and be related to advanced disease [21]. The gene discussed is POU5F1; the disease is colorectal carcinoma.