The tumors in the RGS + IgG showed a statistical difference in tumor growth on a natural log scale (p = 0.002) when compared to the vehicle + IgG control group showed a significant difference in growth, but PDX tumor 3101 was resistant to anti-PD-1 treatment and curiously, anti-PD-1 treatment eliminated the growth inhibitory response to RGS (Figure 7A). The gene discussed is PITX2; the disease is neoplasm.