However, it is surprising that increasing concentrations of RGC above this value inhibit the incidence of invasion/metastasis with decreasing effect (Table 1); a result which is largely mirrored by the immunohistochemical staining for S100A4 in the spleen, colon and primary breast tumours (Supplementary Figures S11–S13) (Table 2). This evidence concerns the gene S100A4 and breast neoplasm.