TGM2 and myopia: This study aims to investigate whether the role of TGM-2 is critical in a mouse model of myopia, the growth-related changes in TGM-2, and its interaction with muscarinic cholinergic receptors (mAChRs) during myopia development; to determine the effect of mAChRs agents on SF derived from TGM-2 knockout (KO) mice; and to identify novel targets for myopia treatment.