SQSTM1 and glioblastoma: Next, to better clarify the action of RDS 3337 in the autophagic process in U87 glioblastoma cells, we verified the autophagic flux using 100 nM BafA1 for 2 h, which prevents lysosomal acidification and, as a consequence, accumulates LC3-II by inhibiting p62/SQSTM1 degradation.