The decreased positivity in the seminiferous tubules with complete preserved spermatogenesis of TGCT patients, compared to non-neoplastic patients, in SALL4, SOX17, RASSF1 and PRSS21 as well as increased CALCA positivity, could be a sign of dysregulated testicular environment due to TGCT or, perhaps, the result of the underlying testicular dysgenesis syndrome in the patients. This evidence concerns the gene RASSF1 and testicular germ cell tumor.