After 48 h of infection, the fluorescence intensity and proportion of 3D4/21 cells transfected with pEGFP-Cl-LDHB were reduced compared with those transfected with the pEGFP-Cl (blank control) vector (Figure 3c), indicating that ASFV replication was decreased when LDHB was overexpressed. This evidence concerns the gene LDHB and infection.