CUX1 and schizophrenia: We quantified synaptic puncta in the cortical neurons with automated high-content imaging analysis by counting the overlap of presynaptic (Bassoon) and postsynaptic (Homer) puncta and found reduced synaptic puncta density in cortical neurons from schizophrenia iPSCs, with a more pronounced reduction in synaptic puncta density in cortical neurons expressing CUX1 (Fig. 1D–F; Additional file 1: Fig. S5).