Although we could not rule out the possibility that the low transduction on this R. malayanus ACE2 by BANAL-20-52 pseudovirions might result from specific ACE2 polymorphism among different R. malayanus bats, the low infection raises the question of whether there might be other bat species as the possible natural host for BANAL-20-52 virus or not, and this notion is further supported by the higher susceptibility among other bat species by BANAL-20-52 (Fig. 3b). Here, ACE2 is linked to infection.