To further explore the role of K70 acetylation of ALDH1L2 on tumor growth and 5-Fu sensitivity in vivo, we injected reconstituted HCT116 cells with ALDH1L2 WT and ALDH1L2 K70Q into nude mice and analyzed their tumorigenesis and 5-Fu sensitivity. This evidence concerns the gene ALDH1L2 and neoplasm.