As reported by Batavani et al. [8] and Bruckmaier et al. [9], an increase in the positive response in the California mastitis test (CMT) is accompanied by increases in sodium, chloride, IGF-1, and immunoglobulin, and decreases in calcium and inorganic phosphorus, showing the presence of tissue damage due to sub-clinical mastitis. This evidence concerns the gene IGF1 and mastitis.