LASSO regression analysis was performed, and six genes (IGF2BP3, ALB, KRT6A, REG3A, KIAA1324, and PAK3) were finally selected (Fig. 3C, D).Then, we used GSE62452 to explore the expression of the six genes we finally screened in cancer tissue and adjacent tissues, and the results showed that except for IGF2BP3, the remaining five genes had abnormal expression (supplementary Table 1). The gene discussed is REG3A; the disease is cancer.