Considering that annexin V presents an affinity for the charged lipid phosphatidylserine at the membrane, flow cytometry experiments were carried out in order to determine if different concentrations of the peptide ΔM4 on HaCaT, A375 melanoma, and A431 cells induce the exposure of this phospholipid, which is indicative of apoptosis. This evidence concerns the gene ANXA5 and melanoma.