The ROCR package in R software (v 4.3.0) was utilized to calculate the AUC values and plot the ROC curves of the hub gene in patients with HNSCC, we found that XIST, TSIX, PAX6, FAM153A, CRYBB2, C1orf51, and C3orf57 exhibited AUC values ranging from 0.5 to 0.7, indicating a relatively lower predictive value. The gene discussed is FAM153A; the disease is head and neck squamous cell carcinoma.