Somatic mutations T cell panel (CD8+ T cells, CD4+ T cells and Treg by Flow Cytometry assesmentthe presence of CD8+PD-1+IL-7Rα−, CD4+PD-1+, and CD4+OX-40+ T cells indicates activated T cells in the tumor.melanoma cells were the predominant cells present in MelTIL024 and MelTIL026checkpoint blockade inhibitoranti-CTLA4 (ipilimumab)anti-PD-1 alone (pembolizumab or nivolumab). The gene discussed is TNFRSF4; the disease is melanoma.