ACSL1 and steatosis: To determine whether ACSL1 is responsible for steatosis in Hmgcs2ΔLiv mice, we incubated the primary hepatocytes from Hmgcs2ΔLiv mice and Hmgcs2F/F mice under lipogenic condition (BSA-conjugated palmitate) in the presence or absence of Triascin C, a specific ACSL1 inhibitor34.