We found no difference between the fasted Hmgcs2F/F and Hmgcs2ΔLiv mice in the expression of TBK1 or its phosphorylated form (Fig S3A), indicating that the mitochondrial TBK1-ASCL1 axis may not be involved in the fasting-mediated hepatic steatosis in Hmgcs2ΔLiv mice. Here, TBK1 is linked to fatty liver disease.