To assess whether tumor cell-intrinsic MCU affected antitumor immune responses, we generated Mcu deficient (Mcu−/−) MC38 murine colorectal tumor cells, Mcu−/− lewis lung carcinoma (LLC) murine lung cancer cells and Mcu−/− B16-OVA murine melanoma cells (Extended Data Fig.3f,p,q). This evidence concerns the gene MCU and lung carcinoma.