Interestingly, during the validation process, we found that JUNB, DUSP1, FOSB, and NR4A1 showed a basically consistent expression trend in NAFLD, HCC, and NAFLD-related HCC data sets; that is, the expression levels in normal control samples were much higher than those in diseased tissues. The gene discussed is NR4A1; the disease is hepatocellular carcinoma.