While MAPK pathway activation was shown to be involved in YAP1 translocation and activation [19, 48, 49, 50, 51] in human malignancies, additional research is required to determine the exact mechanisms involved in YAP1 translocation, and its effect on the transcriptional regulation of EGFR expression in response to trametinib treatment in HNSCC cell lines. Here, YAP1 is linked to head and neck squamous cell carcinoma.