To further explore metabolic modifications, we first analyzed glycolysis in control and DMD hiPSC-fibs using the glycolysis stress test on the Seahorse analyser. DMD hiPSC-fibs showed up-regulated extracellular acidification, glycolytic rate, and glycolytic capacity compared to n control hiPSC-fibs, indicating an important up-regulation of glycolysis in the absence of dystrophin isoforms (Fig. 3A). This evidence concerns the gene DMD and Duchenne muscular dystrophy.