To assess the potential impact that NDD-associated mutations might have on ATP engagement, given that structures are static, we mapped conserved SMARCA2/4 mutant residues onto the open state, ADP bound (similar to apo structure) and onto the closed, ADP-BeFx-bound yeast SNF2 nucleosome bound structures102, which allows mapping of ~85% of all SMARCA2/4-ATPase positions (Extended Data Fig. 4h). Here, SMARCA4 is linked to Neurodevelopmental delay.