It was discovered that roburic acid (RBA) from the extracts of Gentiana macrophylla Pall., a herb for treating RA in Southeast Asia, have strong biological activities such as anti-osteoarthritis, anti-inflammatory, and reliving TNF-related conditions.9–15 However, it has not been tested in RA model yet and its functional mechanism is unclear. Here, TNF is linked to rheumatoid arthritis.