Although circulating levels of the liver enzyme aspartate aminotransferase (AST) were moderately increased, serum alanine aminotransferase (ALT) levels were unaffected (Fig. 10c) and there were no signs of liver fibrosis, as assessed by monitoring for collagen deposition by histology (Picrosirius red) (Fig. 10b). Here, GPT is linked to Hepatic fibrosis.