1 l of log-phase Sf9 insect cells growing in Sf-900 III serum free media (Gibco) were seeded at 3 × 106 cells/ml, and secondary baculoviruses of pFB-BRCA2 or pFB-BRCA2-eGFP were used to infect the cells with a multiplicity of infection equal to 5 for 66 hours (140 rpm, 27°C). Here, BRCA2 is linked to infection.