To determine the impact of Asxl1 on atherosclerosis, we attempted to model Asxl1 CH by transplanting CD45.2+Cas9+ transgenic long-term hematopoietic stem cells (LT-HSCs) infected with control (nontargeting guide RNAs) or Asxl1-G623* guide RNAs mixed with CD45.1+ WT cells into lethally irradiated Ldlr–/– mice. This evidence concerns the gene ASXL1 and atherosclerosis.