To explore the signaling pathways affected by DBF4, we ranked 31425 genes from HCC samples in GEO database (GSE76427) by their relative DBF4 expression and used those in top quarter (DBF4high) and the bottom quarter (DBF4low) for gene set enrichment analyses (GSEAs; Figure S5a). This evidence concerns the gene DBF4 and hepatocellular carcinoma.