Using this neonatal ZIKV infection model, here we examined whether acute ZIKV infection at the time of PNN development affects PNN formation by analyzing the intensity and the area of Wisteria floribunda agglutinin (WFA) staining and the degradation of aggrecan and brevican in the brains of ZIKV-infected mice at 15, 30, and 60 dpi. Here, ACAN is linked to Zika virus infectious disease.