Due to the size of the G6PD gene, located on the X chromosome, four amplicons were used to target five key variants (202, 376, 542, 680, and 968) associated with G6PD deficiency, namely African-type G6PD A−, which results in diminished activity of the enzyme glucose-6-phosphatase dehydrogenase enzyme11,28. The gene discussed is G6PD; the disease is G6PD deficiency.