RAG1 and neoplasm: Next, Rag-1 deficient (Rag-1-/-) mice and NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) mice, both of which lack functional T cells and B cells and exhibit the immuno-deficient phenotype, were used to test the direct influence of GD-NT-mediated pyroptosis on tumor growth in vivo. E0771 cells and HeLa cells stably expressing Dox-inducible GD-NT (E0771-GD-NT cells or HeLa-GD-NT cells) were inoculated in Rag-1-/- mice and NSG mice, respectively.