PRKAA1 and neoplasm: To further test the anti-tumor immunity induced by GD-NT-mediated pyroptosis and the influence of AMPK-mediated phosphorylation, 0.9 ×106 of E0771 parental cells (90% of total inoculated cells to form tumor allograft) mixed with 0.1 × 106 of E0771 GD-NT cells (10% of total inoculated cells to serve as a whole-cell vaccine) were implanted into 4th mammary fat pad in Rag-1-/- mice or wild-type mice.