Consistent with diet-induced perturbations in metabolic health, both C57BL/6J and BXH9 BXH9/TyJ mice fed a WD had increased body weight and adiposity, fasting hyperglycaemia, fasting hyperinsulinemia, glucose intolerance, and lower systemic insulin sensitivity (Matsuda Index) relative to chow-fed controls (Figure 5—figure supplement 1A–E). Here, INS is linked to hyperinsulinism.