In conclusion, the obtained results exhibited that 5-FU nanogel has an excellent effect rather than the 5-FU which was confirmed by suppressing the proliferation of colon cancer via inhibition of the TLR2/ NF-κβ and PI3K/AKT/mTOR as well as lowering the expression levels of Ki-67. Here, NFKB1 is linked to colonic neoplasm.