Additionally, colon cancer tissues treated with 5-FU nanogel demonstrated high expression of caspase-3 more than 70% with strong cytoplasmic and/or nuclear staining (grade 3) which indicates induction of apoptosis and proves the efficiency of the 5-FU nanogel over the 5-FU(Fig. 11IIID). Here, CASP3 is linked to malignant colon neoplasm.