To address whether USP5 could regulate ferroptosis by stabilizing LSH in HCC, we first used gene set enrichment analysis to explore the effects of USP5 on ferroptosis in HCC, and the results revealed that “WikiPathways Ferroptosis,” “reactive oxygen species pathway,” and “iron ion transport” showed enrichment differences between high and low USP5 expression group (Figure 3A). This evidence concerns the gene HELLS and hepatocellular carcinoma.