Meanwhile, a series other assays used for adjuvant therapy were sprang up, such as Guardant 360 CDx assay (Guardant Health) based on simultaneous mutation detection of 55 tumor genes for pan‐cancer, and therascreen PIK3CA RGQ PCR Kit (QIAGEN Manchester) based on PIK3CA mutations for BC. The gene discussed is PIK3CA; the disease is breast cancer.