12 novel epigenetic markers (JAK3, RASGRF1, CPXM1, SHF, DNM3, CAV2, HOXA10, B3GNT5, ST3GAL6, DACH1, P2RX3, and chr8:23572595) for detecting BC and four internal control markers (CREM, GLYATL3, ELMOD3, and KLF9). The gene discussed is SHF; the disease is breast cancer.