CDH1 and cancer: The Estrogen-dependent changes in the AJ directly influence cell stiffness and motility, two functions that are highly relevant for cancer progression, in particular in respect to progression from benign to malignant tumors or metastasis and address the relevant endpoints of phenotypic changes-migration and nuclear receptor activation at the same time By applying a cytoplasmic stain on the parental cells or by using an E-Cadherin-EGFP-expressing reporter cell line (de Beco et al., 2009; Klutzny et al., 2022), the appearance of the plasma membrane can be quantified by high content imaging.