Moreover, consistent with previous reports that increased ACO1 led to excess accumulation of ROS and cell death in cancer cells (16, 17), knockdown of ENO1 led to increased cell death (FigureS 14E, F), enhanced lipid ROS generation (Figures 15A, B), and increased accumulation of intracellular ferrous ion (Figure 15C), particularly in the presence of iron. This evidence concerns the gene ACO1 and cancer.