Having discovered in cell culture that septin 11 regulates endothelial activation by 27HC, its impact on atherosclerosis was determined in control floxed septin 11 mice (sept11fl/fl) and mice deficient in septin 11 in endothelial cells (sept11∆EC) on apoE-/- background (Supplementary Fig. 3f–i). This evidence concerns the gene APOE and atherosclerosis.