In addition, in our study, the expression of SPP1 was up-regulated in CAVD tissues, but down-regulated in osteogenesis induced VICs, and stable in VECs with shear stress, indicating that the expression of SPP1 varied in different cells and with different interventions, and the mechanism of its participation in CAVD was complex. This evidence concerns the gene SPP1 and congenital bilateral aplasia of vas deferens from CFTR mutation.