After independent data sets and qRT-PCR verification, a hsa-circ-0073813/hsa-circ-0027587–hsa-miR-525-5p–SPP1/HMOX1/CD28 network was finally constructed, which contribute to a deeper understanding of CAVD and provide possible targets for the treatment of CAVD in the future. This evidence concerns the gene CD28 and congenital bilateral aplasia of vas deferens from CFTR mutation.