Regarding TNFα signaling via NF-κB in endothelial cells, in animal studies, Pecam1 KO mouse developed nonalcoholic steatohepatitis (Goel et al, 2007), whereas Tlr4 KO mouse (Zhang et al, 2020) and Il15 KO mouse (Cepero-Donates et al, 2016) were protected from NAFLD. This evidence concerns the gene NFKB1 and metabolic dysfunction-associated steatohepatitis.