TP53 and acute kidney injury: In addition, western blotting showed that the protein expressions of AQP1, P53 and P21 in rat renal tissue increased significantly and reached a peak at 12h after LPS injection and then decreased at other time periods (24h, 48h, 72h, 7d) during the course of endotoxin-induced AKI (Fig 4E), which consistent with the levels of AQP1 in serum.