TRERF1 and parasitic infectious disease: To further investigate the function of PI-PLC over time, we also performed an experiment, in which we added Rapa not only to young rings (3 hpi) but also to later rings (14 hpi), trophozoites (24 hpi), and late schizonts (40 hpi), followed by measuring the parasitemia in the subsequent intraerythrocytic parasite cycle.