APP and Alzheimer disease: To date, the most widely used line is the 5xFAD mouse line, which carries the mutations in human genes associated with the familial from of AD, including five mutations in the APP and presenilin1 (PSEN1) human transgenes (APP KM670/671NL (the Swedish mutation), APP I716V (the Florida mutation), APP V717I (the London mutation), and PSEN1 (M146L, PSEN1 L286V) which contribute to the rapid development of amyloidosis.