Mutations in genes such as PIK3CA, phosphatase and tension homologues (PTEN), AKT1, and tuberous sclerosis 1/2 (TSC1/2) can all lead to dysregulation of signalling pathways, which can drive important physiological processes such as tumour metabolism, proliferation, angiogenesis, and metastasis.14–17. This evidence concerns the gene TSC1 and neoplasm.